HRP-Juice, two bottle
Our stabilized ultra-sensitive Luminol based substrates detect horseradish peroxidase at femtogram level and our chemiluminescent systems 3 to 5 more times sensitive than our best competitor. These reagents are stable for 21 days after mixing when stored at 4°C.
Storage: Store the reagent bottles, HRP-A and HRP-B, at 4-8 °C .
Usage: Mix equal volumes of HRP-A and HRP-B in a clean container and equilibrate at room temperature for 30 minutes before use. Do not contaminate the HRP enzyme substrate with HRP enzyme or other proteins.HRP enzyme substrate has a wide range to detect HRP enzyme in solution as well as in ELISA. The lower enzyme concentration may be a few femto grams and the highest concentration HRP of enzyme may be one nano gram depending on the source of the HRP enzyme.
For good results, wash your tube or micro titer plate or membrane with 0.2M phosphate buffer, pH 8.4 to 8.6, and then use the substrate. Best results for chemiluminescence can be obtained from 2 minutes to 20 minutes after contacting substrate with HRP enzyme.