Gaussia Vectors

pUC18-Gluc

pUC18 Vektor with Gaussia princeps Luciferase, expressing native Gaussia luciferase without the secretion signal (SS) with 6His-tag, cloned in BamHI and XbaI sites, 3214 bp

pUC18 SS Gluc

pUC18 Vektor with Gaussia princeps Luciferase, expressing native Gaussia luciferase with the N-terminal secretion signal (SS) and with 6His-tag, cloned in BamHI and XbaI sites, 3214 bp

pGluc Basic1

A promoterless vector with a MCS site upstream of the humanized Gaussia luciferase coding sequence (with secretion signal +SS). This vector is designed for promoter analysis and will express secreted Gaussia luciferase:


Features:

  •     T7 promotor bases: 20-38
  •     Polylinker bases: 12-63
  •     Gaussia luciferase reporter gene: 63-653
  •     SP6 promotor: 669-686
  •     Synthetic polyadenylation site: 653-697
  •     SV40 promotor bases: 1253-1573
  •     SV40 origin of replication: 1352-1437
  •     SV40 PolyA: 2458-2830
  •     Co1E1 origin: 3090-3763
  •     Neomycin-resistance gene: 1609-2403
  •     Ampicillin-resistance gene: 3908-4768

pGluc KDEL Basic

Humanized Gaussia Luciferase, promoterless; with N-terminal secretion signal (SS), designed for insertion of your own promoters, comes with C-terminal KDEL endoplasmic retention signal for intracellular expression:

Features:

  •     Polylinker bases: 12-63
  •     Gaussia luciferase gene(566 bp): 76-642
  •     SP6 promoter: 681-698
  •     Synthetic polyadenylation site: 665-709
  •     SV40 promoter bases: 1265-1585
  •     SV40 origin of replication: 1364-1449
  •     Neomycin ORF: 1621-2415
  •     SV40 PolyA: 2470-2842
  •     ColE1 origin: 3102-3775
  •     Ampicillin ORF: 3920-4780

pCMV Gluc1

Humanized Gaussia luciferase with N-terminal secretion signal (SS) . This positive control vector is very useful in evaluating the efficiency of transgene expression using Gaussia luciferase as a reporter. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E. coli and can be used to establish stable cell lines expressing Gaussia luciferase:

Features:

  •     CMV promoter bases: 209-863
  •     Gaussia luciferase gene: 920-1497
  •     T7 promoter bases: 864-882
  •     Polylinker bases: 889-907
  •     SP6 promoter: 1513-1530
  •     Synthetic polyadenylation site: 1497-1541
  •     SV40 promoter bases: 2082-2417
  •     SV40 origin of replication: 2196-2281
  •     Neomycin ORF: 2453-3247
  •     SV40 PolyA: 3302-3674
  •     ColE1 origin: 3934-4607
  •     Ampicillin ORF: 4752-5612

pCMV KDEL Gluc1

Humanized Gaussia luciferase, with N-terminal secretion signal (SS), with C-terminal KDEL endoplasmic reticulum retention signal for intracellular expression. The KDEL sequence is an endoplasmic reticulum retention signal which causes retention of the Gaussia luciferase in the endoplasmic reticulum and results in high levels of intracellular luciferase expression:


Features:

  •     CMV promoter bases: 209-863
  •     Gaussia luciferase gene: 920-1486
  •     T7 promoter bases: 864-882
  •     Polylinker bases: 889-907
  •     Synthetic polyadenylation site: 1509-1553
  •     SV40 promoter bases: 2094-2429
  •     SV40 origin of replication: 2208-2293
  •     Neomycin ORF: 2465-3259
  •     SV40 PolyA: 3314-3686
  •     ColE1 origin: 3946-4619
  •     Ampicillin ORF: 4764-5624

pCMV Gluc minus SS

Humanized Gaussia luciferase without secretion signal (SS). This positive control vector is very useful in evaluating the efficiency of intracellular transgene expression using Gaussia luciferase as a reporter. Also, this vector is also a helpful tool for fusing Gluc to a specific protein of interest. This vector has both Ampicillin resistance and Neomycin resistance. Therefore it can be easily propagated in E.coli and used to establish stable cell lines expressing Gaussia luciferase:

Features:

  •     CMV promoter bases: 209-863
  •     Gaussia luciferase gene without the signal peptide: 915-1424
  •     T7 promoter bases: 864-882
  •     Polylinker bases: 889-907
  •     Synthetic polyadenylation site: 1443-1487
  •     SV40 promoter bases: 2028-2363
  •     SV40 origin of replication: bases 2142-2227
  •     Neomycin ORF: 2399-3193
  •     SV40 PolyA: 3248-3620
  •     ColE1 origin: 3880-4553
  •     Ampicillin ORF: 4698-5558