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Firefly Assays

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    Beetle-Juice

    The Beetle-Juice from PJK is an optimal buffer system for measuring Firefly luciferase...

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    D-Luciferin

    p.a. free acid, Firefly (synth.) D-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic]; C11H8N2O3S2, MW 280.33g/mol; CAS [2591-17-5], p.a.quality, purity: >99%.

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    Coenzyme A

    free acid, CAS [85-61-0], yellowish lyophilised powder; Coenzyme A, Red: approx. 85% enzymatically measured with phosphotransacetylase.

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    Coelenterazine

    nativ (synth.) substrate for Renilla and Gaussia luciferase. [2-(p- hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzyl-imidazol[1,2-a]pyrazin-3-(7H)-one...

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Beetle-Juice KIT

The Beetle-Juice from PJK is an optimal buffer system for measuring Firefly luciferase in a wide range of in vitro applications. The assay shows constant light signal in the range of five to fifteen minutes.

Beetle Long Glow-Juice

The Beetle Long GLOW-Juice from PJK was optimized for measuring Beetle luciferase in high throuhgput screenings (HTS). The buffer shows constant light signal in the range of five hours.

Beetle Lysis-Juice One-Step Luciferase Assay Firefly

The Beetle Lysis-Juice from PJK is an optimal buffer system for measuring Beetle luciferase including cell lysis in one step in a wide range of in vitro applications. The assay shows constant light signal in the range of five to fifteen minutes.

2xLysis-Juice

Lysis-Buffer with detergence dual concentrated.

Coenzyme A

free acid, CAS [85-61-0], yellowish lyophilised powder; Coenzyme A, Red: approx. 85% enzymatically measured with phosphotransacetylase. Coenzyme A assay, spectralphotometric; approx. 89.5% at 260 nm, water: Karl Fischer: approx. 2.9%; MW 767.54 g/mol.Store dry and dark at -20°C.

D-Luciferin

p.a. free acid, Firefly (synth.) D-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic]; C11H8N2O3S2, MW 280.33g/mol; CAS [2591-17-5], p.a.quality, purity: >99%. Analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C

D-Luciferin-Methylester

Firefly (synth.), D-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic-methylester]; C12H10N2O3S2, MW 294.35 g/mol; CAS [2591-17-5], p.a. quality, purity: >98%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C.

 

D-Luciferin-NA-Salt

Firefly (synth.), D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-delta-2-thiazolene-4-carboxylic acid, sodium salt; C11H7N2NaO3S2, MW 302.307 g/mol, CA[103404-75-7], p.a. quality, purity: >99%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C

D-Luciferin-K-Salt

Firefly (synth.), D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-delta-2-thiazolene-4-carboxylic acid, potassium salt; C11H7KN2O3S2, MW 318.415 g/mol; CAS[115144-35-9] p.a. quality, purity: >99%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C

L-Luciferin

p.a. free acid, L-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid]; C11H8N2O3S2, MW 280.325g/mol, CAS [2591-17-5] p.a. quality, purity: >99% L-form. Analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under Argon. Store at -20°C

ATP

(Adenosintriphosphat), C10H16N5O13P3, MW: 507,181 g/mol. CAS [56-65-5], Store at +4°C..

Additional Informations

ATP is a multifunctional nucleotide, the most significant function of which in cell biology is as a "molecular currency" of intracellular energy transfer. In this role, ATP transports chemical energy within cells for metabolism. It is produced as an energy source during the processes of photosynthesis and cellular respiration and consumed by many enzymes and a multitude of cellular processes including biosynthetic reactions, motility and cell division. In signal transduction pathways,ATP is used as a substrate by kinases that phosphorylate proteins and lipids, as well as by adenylate cyclase, which uses ATP to produce the second messenger molecule cyclic AMP

DTT

(1,4 Dithiothreitol), Cleland´s Reagent, CHR (SH) > 99%; C4H10O2S2; MW:154.25 g/mol, CAS [3483-12-3], Store at +4°C.

Additional Informations

Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's formula is C4H10O2S2 and the molecular structure of its reduced form is shown at the right; its oxidized form is a disulfide-bonded 6-membered ring (shown below). Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE). DTT is an unusually strong reducing agent, owing to its high conformational propensity to form a six-membered ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions and is illustrated below. The intermediate mixed-disulfide state is unstable (i.e., poorly populated) because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond. The reducing power of DTT is limited to pH values above ~7, since only the negatively charged thiolate form -S– is reactive (the protonated thiol form -SH is not); the pKa of thiol groups is typically ~8.3.