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Enzyme-linked immunosorbent assay (ELISA) is a typical technique to identify a molecule (antigen) by specific antibody.
Horseradish peroxidise enzyme is the most widely used in EIA and EIH. Typical peroxidases are hemoproteins and transfer hydrogen from hydrogen donors to hydrogren peroxide.
Alkaline phosphatase is used in many methods like Immunoassays and Reportergene Assays.