Sirius Single Tube Luminometer
Sirius can be employed according to the individual needs of each user. In its basic configuration the luminometer is ready for standard measurements. Sirius can be upgraded to form a powerful measurement system for any luminescence application. The Sirius luminometer measures standard glow chemistry of single samples from a variety of sample formats. For flash chemistry, and to make operation more convenient, the instrument can be formatted with one or two built-in automatic reagent injectors. In stand-alone mode, the built-in microprocessor software supports four protocols for data evaluation, including background subtraction capabilities and replicate management, as well as printer output. Modular PC-Software further enhances performance by organizing the measurement dialog and data evaluation.
Sirius is the perfect choice for all luminescent flash and glow applications performed in single sample format, for example:
- Reporter gene assays, including dual-reporter assays
- Cell proliferation, cytotoxicity and biomass assays
- Apoptosis assay
- ATP assays
- Ca2+ measurements and aequorin-based assays
- Cellular luminescence/reactive oxygen species
- Environmental toxicity and mutagenicity assays
Photon Counting Detecton
Sirius Luminometer features advanced photoncounting electronics along with a highly sensitive detector. It provides the lowest signal background and highest linearity covering 6 decades. No cumbersome internal calibration is required and no gain switch is needed.
Light collection is maximized through both the proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. As a result, reporter gene assays using firefly luciferase can now detect less than 1000 molecules of the enzyme with the Sirius Luminometer.
Injection System
Sirius is available with up to two high-precision reagent injectors. Variable injection volumes can be set from 20 to 300 μl. Flexible speed settings and precise injector positioning allow homogenous mixing of reagents for every type of sample.
Short reagent lines and the back-pumping option guarantee the most economical utilization of valuable reagents. Sirius is equipped with reliable, bellow-type pumps. Selection of chemically inert materials assures the injectors are virtually maintenance-free. Sirius will detect presence of a sample vial prior to injection, to avoid unintended injection into an empty sample chamber.
Stand-alone Operation
Sirius is equipped with on-board microprocessor software for instrument control, data calculation and printer output. In stand-alone mode Sirius supports four protocols designed to meet the needs of state of the art luminescence assays.
In addition to the Raw Data protocol, which allows standard measurements in a row, Single Assay, Dual Assay and Cut-off Assay are available, supporting predefined measurement sequences, data evaluation and printer output.
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