DNA Polymerase

PJK´s PCR reagents includes a wide

range of DNA Polymerases.

Taq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme possesses a highly processive 5'-3' DNA polymerase activity with optimum activity achieved at 74°C. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). Taq DNA Polymerase lacks 3'-5' exonuclease activity.

Red Taq DNA Polymerase contains an inert red dye which allows on line monitoring of the electrophoresis and identification of the reactions which contain enzyme. The dye has no adverse effect on PCR.

Hot Start DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme possesses a highly processive 5'-3' DNA polymerase activity with optimum activity achieved at 74°C. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95°C). Hot Start DNA Polymerase lacks 3'-5' exonuclease activity.

Pfu DNA Polymerase is a highly purified recombinant highly thermostable DNA polymerase that has been isolated from E. coli carrying a vector encoding the Pyrococcus furiosus DNA polymerase gene. The enzyme is twenty-five times more accurate than Taq DNA Polymerase and it produces blunt-end amplicons.

High Fidelity DNA Polymerase is a blend of DNA polymerases specially designed for the highly accurate and efficient amplification of fragments up to 30kbp including GC-rich or other difficult templates.

Red Hot Start DNA Polymerase contains an inert red dye which allows on line monitoring of the electrophoresis and identification of the reactions which contain enzyme. The dye has no adverse effect on PCR

Click here for Basic Protocol DNA Polymerases

go to pjk-shop: