Firefly-Assays
Beetle-Juice
The Beetle-Juice from PJK is an optimal buffer system for measuring Firefly lucifarase in a wide range of in vitro applications. The assay shows constant light signal in the range of five to fifteen minutes.
Beetle LongGLOW-Juice
The Beetle Long Glow-Juice from PJK was optimized for measuring Beetle lucifarase in high througput screenings (HTS). The buffer shows constant light signal in the range of five hours.
Beetle Lysis-Juice
The Beetle Lysis-Juice from PJK is an optimal buffer system for measuring Beetle luciferase including cell lysis in one step in a wide range of in vitro applications. The assay shows constant light signal in the range of five to fifteen minutes.
2xBeetle Lysis-Juice
The 2xBeetle Lysis-Juice from PJK is an optimal buffer system for measuring Beetle luciferase including cell lysis in one step in a wide range of in vitro applications. The assay shows constant light signal in the range of five to fifteen minutes.
Substrates for Assays
High qualtity substrates are the basis for exact results in enzyme based bioluminescent assays. PJK`s substrates stands for high purity for best results and light output.
Schema of Firefly luciferase reaction. Oxidation of D-Luciferin to Oxiluciferin
in presence of ATP, oxygen and meallic ions.o and in vivo applications.
Luciferin is the specific substrate for Firefly luciferase in gene reporter assays. PJK´s high qualitiy Luciferin can used for firefly assays in a wide range of in vitro and in vivo applications.
D-Luciferin
p.a. free acid, Firefly (synth.) D-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic]; C11H8N2O3S2, MW 280.33g/mol; CAS [2591-17-5], p.a.quality, purity: >99%. Analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C
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D-Luciferin-Methylester
Firefly (synth.), D-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic-methylester]; C12H10N2O3S2, MW 294.35 g/mol; CAS [2591-17-5], p.a. quality, purity: >98%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C.
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D-Luciferin-NA-Salt
Firefly (synth.), D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-delta-2-thiazolene-4-carboxylic acid, sodium salt; C11H7N2NaO3S2, MW 302.307 g/mol, CA[103404-75-7], p.a. quality, purity: >99%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C
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D-Luciferin-K-Salt
Firefly (synth.), D-(-)-2-(6'-hydroxy-2'-benzothiazolyl)-delta-2-thiazolene-4-carboxylic acid, potassium salt; C11H7KN2O3S2, MW 318.415 g/mol; CAS[115144-35-9] p.a. quality, purity: >99%, analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under argon. Store at -20°C
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L-Luciferin
p.a. free acid, L-[4,5 Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid]; C11H8N2O3S2, MW 280.325g/mol, CAS [2591-17-5] p.a. quality, purity: >99% L-form. Analytical certificate by chiral HPLC analysis according to product information. Packed in brown glass ampoules, bottled under Argon. Store at -20°C
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Coenzyme A
free acid, CAS [85-61-0], yellowish lyophilised powder; Coenzyme A, Red: approx. 85% enzymatically measured with phosphotransacetylase. Coenzyme A assay, spectralphotometric; approx. 89.5% at 260 nm, water: Karl Fischer: approx. 2.9%; MW 767.54 g/mol.Store dry and dark at -20°C.
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ATP
(Adenosintriphosphat), C10H16N5O13P3, MW: 507,181 g/mol. CAS [56-65-5], Store at +4°C..
Additional Informations
ATP is a multifunctional nucleotide, the most significant function of which in cell biology is as a "molecular currency" of intracellular energy transfer. In this role, ATP transports chemical energy within cells for metabolism. It is produced as an energy source during the processes of photosynthesis and cellular respiration and consumed by many enzymes and a multitude of cellular processes including biosynthetic reactions, motility and cell division. In signal transduction pathways,ATP is used as a substrate by kinases that phosphorylate proteins and lipids, as well as by adenylate cyclase, which uses ATP to produce the second messenger molecule cyclic AMP
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DTT
(1,4 Dithiothreitol), Cleland´s Reagent, CHR (SH) > 99%; C4H10O2S2; MW:154.25 g/mol, CAS [3483-12-3], Store at +4°C.
Additional Informations
Dithiothreitol (DTT) is the common name for a small-molecule redox reagent known as Cleland's reagent. DTT's formula is C4H10O2S2 and the molecular structure of its reduced form is shown at the right; its oxidized form is a disulfide-bonded 6-membered ring (shown below). Its name derives from the four-carbon sugar, threose. DTT has an epimeric ('sister') compound, dithioerythritol (DTE). DTT is an unusually strong reducing agent, owing to its high conformational propensity to form a six-membered ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reactions and is illustrated below. The intermediate mixed-disulfide state is unstable (i.e., poorly populated) because the second thiol of DTT has a high propensity to close the ring, forming oxidized DTT and leaving behind a reduced disulfide bond. The reducing power of DTT is limited to pH values above ~7, since only the negatively charged thiolate form -S– is reactive (the protonated thiol form -SH is not); the pKa of thiol groups is typically ~8.3.
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